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KMID : 0613820100200070991
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Journal of Life Science
2010 Volume.20 No. 7 p.991 ~ p.998
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Cobalt Chloride-Induced Down-Regulation of Puromycin-Sensitive Aminopeptidase Involved in Apoptosis of PC-3 Cells
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Lee Suk-Hee
Kim Hwan-Gyu
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Abstract
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Hypoxia is an indicative of pro-apoptotic and anti-apoptotic biphasic effects, which appear to be dependent upon the cell type and the condition of the cells. The hypoxia-mimetic agent, cobalt chloride (CoCl₂), has been shown to induce apoptosis in a variety of cell types, but the mechanism by which this occurs has yet to be thoroughly elucidated. Puromycin-sensitive aminopeptidase (PSA) gene was decreasingly expressed in response to CoCl₂. In this report, puromycin pretreatment applied to PC-3 cells resulted in apoptosis. To determine whether PSA is involved in apoptosis, we examined the apoptotic properties of the PC-3 cells after siRNA knockdown of PSA. PSA siRNA-induced PSA silencing revealed that endogenous PSA may be involved in apoptosis of the PC-3 cells. These results indicated that PSA may perform a vital function in cell survival of the PC-3 cells.
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KEYWORD
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Cobalt chloride, puromycin-sensitive aminopeptidase(PSA), apoptosis, puromycin
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